Development of stably transfected cell lines to screen Endocrine Disrupters ER-EcoScreen assayTM and AR-EcoScreen assayTM (Stable CHO clones containing luciferase based reporter gene and expressing hormone receptors)

INTRODUCTION We have developed genetically engineered stable transfected cell lines, expressing hormone receptor and luciferase based reporter genes, for screening compounds and compound mixtures for endocrine disrupter activity. We have named the lines " ER-EcoScreen™ " (expressing estrogen receptor) and " AR-EcoScreen™ " (expressing androgen receptor). To establish these cell lines we introduced the plasmids used in our transient transfection Eco-Screen Assay™ systems. We have demonstrated that the cells have the same reactivity to the samples tested in the Eco-Screen Assay™ system. We also have confirmed that these cell lines do not lose reporter activity during continuous cell passage.